Automation and Basic Techniques in Medical Microbiology by Santi M. Mandal & Debarati Paul

Author:Santi M. Mandal & Debarati Paul
Language: eng
Format: epub
ISBN: 9781071623725
Publisher: Springer US

8.4.1 Automated Validation of Every Result (VITEK) System for Microbial Identification

In the last 30 years, various automated systems have been developed to facilitate identification of microbes and also for carrying out antimicrobial susceptibility tests (AST) after interpreting the results on the automated system. These tests are performed on a micro-dilution tray, and after overnight incubation they are spectrophotometrically analysed, and data is recorded (Stager and Davis 1992). In the 1970s, the VITEK platform was found for automated identification and AST analysis which evolved further to the present form VITEK 2 system, and this can automatically perform all steps involved in identification and AST. The initial manual step required is preparation of a primary inoculum and standardization of process (Funke et al. 1998). This new system (Fig. 8.4) allows kinetic analyses by recording data from every test after 15-min interval. A system developed by combining an optical system with multi-channel photometer and fluorimeter is “API 20 Strep” for members of the Streptococcaceae family. Other tests developed include serological typing of the streptococci with beta-haemolytic ability, coagulase test with Staphylococcus aureus, optochin susceptibility test for S. pneumonia and yellow pigment formation plus motility test for enterococci.

Fig. 8.4VITEK 2 Compact system with cards (a, b) and the instrument. This system allows kinetic analyses by recording data for every test after intervals of 15 min. The optical system records turbidity, fluorescence and colorimetric signal by combining multi-channel photometer and fluorimeter readings

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